Electrophoresis Gel Stains
Gold Biotechnology Inc Coomassie Brilliant Blue G 250
Coomassie Brilliant Blue G-250 (CBBG) is a member of the Coomassie family and is used as a common analytical dye in SDS-PAGE and BN-PAGE CBBG can also be used with the Bradford Method to determine protein concentration
Lonza Walkersville AdipoRed Assay Reagent, 20 mL
The AdipoRed Assay Reagent is designed for assessing the effect of compounds on the differentiation of preadipocytes or on lipid utilization in mature adipocytes. The lipophilic AdipoRed Assay Reagent specifically partitions into the fat droplets of differentiated adipocytes and fluoresces at 572 nm.This objective, high-throughput, homogeneous fluorescence-based assay quantifies the accumulation of intracellular triglycerides and provides significant advantages to drug discovery efforts in the field of obesity and diabetes.Benefits:Convenient - Simply remove cell culture medium, add premixed reagent and read in a standard fluorometerFast- Process an entire 96-well or 384-well plate in as little as 20 minutesEffective - Provides objective, high throughput measurement of the accumulation of intracellular triglycerides, with high signal to noise ratiosContent:5 x AdipoRed Assay Reagent, 4 mL
Vector Laboratories Peanut Agglutinin (PNA) Rhodamine-labeled 5 mg
Peanut agglutinin (PNA) binds the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates and other soluble and membrane-associated glycoproteins and glycolipids. With exceptions, the PNA receptor sequence is normally sialylated, which prevents binding to its receptor oligosaccharide (see Jacalin). Sialic acid not bound to receptor sugars may also inhibit binding, and including calcium in diluents can enhance binding. This rhodamine-labeled PNA features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 575 nm, emission 600 nm). It is supplied as a solution essentially free of unconjugated fluorophores.
Gold Biotechnology Inc GelRedTM Nucl Acid Gel Stain
GelRed is an ultra-sensitive extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA ssDNA or RNA in agarose gels or polyacrylamide gels GelRed is far more sensitive than EtBr without requiring a destaining step GelRed and EtBr have virtually the same spectra so you can directly replace EtBr with GelRed without changing your existing imaging system
Biotium PAGE GelRed Nucleic Acid Gel Stain, 10,000X in Water
PAGE GelRed is a non-toxic, non-mutagenic red DNA gel stain specifically designed to stain DNA in polyacrylamide gels. PAGE GelRed can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter. While PAGE GelRed also can be used to stain DNA in agarose gels, Biotium’s original GelRed nucleic acid gel stain is more sensitive for agarose gel applications. PAGE GelRed can be removed from DNA after agarose gel staining using commonly available gel extraction kits. PAGE GelRed is 6-8 times more sensitive for dsDNA over RNA. Properties: Liquid; Ex/Em= 308, 551/625 nm (with dsDNA); Store at room temperature and protect from light.
Biotium DMAO, 2mM in DMSO
DMAO is a membrane-permeable DNA dye, excellent for staining live bacteria. Stained cells can be detected by fluorescence microscopy or flow cytometry. DMAO preferentially binds to dsDNA with green fluorescence (Ex/Em, DNA-bound = 496/528 nm). The dye is suitable for both gram-positive and gram-negative strains. Unlike the SYBR dyes, DMAO is extremely stable and can be routinely handled and stored at room temperature without degradation. Properties: Liquid; Store at room temperature or below, protect from light; Ex/Em = 496/528 nm (with DNA).
Biotium GelGreen Nucleic Acid Gel Stain, 10, 000X in Water, 10 mL
GelGreen is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen is far more sensitive than SYBR Safe. Unlike SYBR dyes, which are known to be unstable, GelGreen is very stable, both hydrolytically and thermally. GelGreen is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as blue LED light box, 488 nm laser-based gel scanner, or Dark Reader). GelGreen can be used for either post-electrophoresis gel staining or precast gel staining. Properties: Liquid; Store at room temperature and protect from light.
Biotium Hoechst 33258, 10mg/mL in H2O
Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains. Hoechst 33528 is slightly more water soluble than Hoechst 33342. The dyes can be used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required. Properties: Yellow solution; Store at 4°C, protect from light; Ex/Em= 352/461 nm (with DNA); C25H27Cl3N6O; MW: 533.88; [23491-45-4].
Biotium Hoechst 33258
Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains. Hoechst 33528 is slightly more water soluble than Hoechst 33342. The dyes can be used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required. Properties: Yellow solid; Store dessicated at -20°C, protect from light; Ex/Em= 352/461 nm (with DNA); C25H27Cl3N6O; MW: 623.96; [23491-45-4].
Biotium NucSpot 470, 1000X in DMSO
NucSpot 470 is a cell membrane-impermeant green fluorescent DNA stain. It is virtually non-fluorescent in the absence of DNA, but fluoresces bright green upon DNA binding. While other green nucleic acid stains like TOTO, TO-PRO, or SYTOX dyes stain both the nucleus and cytoplasm, NucSpot 470 specifically stains the nucleus of fixed and permeabilized cells. It also can be used to selectively stain dead cells in living cultures. NucSpot 470 has an excitation/emission of 460/546 nm and can be imaged using standard settings for FITC. Properties: Ex/Em= 460/546 nm (with DNA); Store at 4°C and protect from light.